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简答题对一双链DNA而言,若一条链中(A+G)/(T+C)=0.7,则:(1)互补链中(A+G)/(T+C)=? (2)在整个DNA分子中(A+G)/(T+C)=? (3)若一条链中(A+T)/(G+C)=0.7,则互补链中(A+T)/(G+C)=? (4)在整个DNA分子中(A+T)/(G+C)=?
  • (1)设DNA的两条链分别为α和β,那么:
    A=βT,Tα=Aβ,Gα=Cβ,:Cα=Gβ,
    因为,(Aα+Gα)/(Tβ+Cβ)=(Aα+Gα)/(Aβ+Gβ)=0.7
    所以,互补链中(Aβ+Gβ)/(Tβ+Cβ)=1/0.7=1.43
    (2)在整个DNA分子中,因为A=T,G=C,
    所以,A+G=T+C,(A+G)/(T+C)=1
    (3)假设同(1),则
    Aα+Tα=Tβ+Aβ,Gα+Cα=Cβ+Gβ,
    所以,(Aα+Tα)/(Gα+Cα)=(Aβ+Tβ)/(Gβ+Cβ)=0.7
    (4)在整个DNA分子中
    (Aα+Tα+Aβ+Tβ)/(Gα+Cα+Gβ+Cβ)=2(Aα+Tα)/2(Gα+Cα)=0.7
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